pcDNA-dCas9-FLp300
(Plasmid #61356)

• Purpose: encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
• Depositing Lab: Charles Gersbach
• Publication: Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen; Life Technologies
• Total vector size (bp): 16863
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414)
• Species: H. sapiens (human), Synthetic; S. pyogenes
• Insert Size (bp): 11526
• Mutation: D10A; H840A in S.pyogenes Cas9
• GenBank ID: WP_010922251.1 NP_001420.2
• Entrez Gene: EP300 (a.k.a. KAT3B, MKHK2, RSTS2, p300)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • SV40 NLS (N terminal on insert)
  • SV40 NLS (C terminal on insert)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV F
• 3′ sequencing primer: Bgh R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry