Skip to main content
Addgene

pDN34
(Plasmid #61343)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61343 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 6819
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Promoter CMV
  • Tags / Fusion Proteins
    • PKIt NES (N terminal on insert)
    • GS linker-AsLov2-biNLS2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDN34 was a gift from Barbara Di Ventura & Roland Eils (Addgene plasmid # 61343 ; http://n2t.net/addgene:61343 ; RRID:Addgene_61343)

  • For your References section:

    Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells. Niopek D, Benzinger D, Roensch J, Draebing T, Wehler P, Eils R, Di Ventura B. Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404. 10.1038/ncomms5404 PubMed 25019686
Related items:
Commonly requested with: