pDB22
(Plasmid #61342)

• Purpose: Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)
• Depositing Labs: Barbara Di Ventura, Roland Eils
• Publication: Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.1038/ncomms5404.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Total vector size (bp): 6780
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Synthetic
• Promoter: CMV
• Tags / Fusion Proteins:
  • PKIt NES (N terminal on insert)
  • GS linker-AsLov2-cMyc1A NLS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Supplemental Documents:
  • pDB22 genbank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the mammalian selection marker in the vector backbone for this plasmid has not been verified by sequencing.