pcDNA3-FLAG-LplA(AAG)
(Plasmid #61307)

• Purpose: Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression
• Depositing Lab: Alice Ting
• Publication: Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4551-9. doi: 10.1073/pnas.1404736111. Epub 2014 Oct 13.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5190
• Total vector size (bp): 6558
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E. coli lipoic acid ligase
• Alt name: LpIA
• Species: E.coli
• Insert Size (bp): 1038
• Mutation: E20A, F147A, H149G
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • T7 tag (N terminal on insert)
  • Xpress epitope (N terminal on insert)
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • Ape file

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry