pVHD
(Plasmid
#61303)
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Purpose(Empty Backbone) Vanillate-inducible gene expression plasmid for Sphingomonas melonis Fr1; destination vector for Gateway cloning, allowing C-terminal fusion of proteins to HA tag
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61303 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCM62
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Backbone manufacturerChris Marx
- Backbone size (bp) 6878
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Vector typeBacterial Expression
- Promoter PV10
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Selectable markerschloramphenicol (in Gateway cassette)
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Tetracycline, 25 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVHD was a gift from Julia Vorholt (Addgene plasmid # 61303 ; http://n2t.net/addgene:61303 ; RRID:Addgene_61303) -
For your References section:
Synthetic vanillate-regulated promoter for graded gene expression in Sphingomonas. Kaczmarczyk A, Vorholt JA, Francez-Charlot A. Sci Rep. 2014 Sep 29;4:6453. doi: 10.1038/srep06453. 10.1038/srep06453 PubMed 25262659
