pBluescript alpha-SSeCKS
(Plasmid
#61295)
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Purposecontains rat alpha SSeCKS cDNA
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61295 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II SK(+)
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 9000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namealpha SSeCKS
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Alt nameA kinase (PRKA) anchor protein 12
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SpeciesR. norvegicus (rat)
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Insert Size (bp)6300
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MutationcDNA with 1kb of 3' UTR and 150bp of 5' UTR
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Entrez GeneAkap12 (a.k.a. AKAP12A, AKAP12B, AKAP12G)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7, M13-F20
- 3′ sequencing primer T3, M13-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: Addgene's quality control sequencing finds a single nucleotide mismatch in the alpha-SSeCKS 3' UTR
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBluescript alpha-SSeCKS was a gift from Irwin Gelman (Addgene plasmid # 61295 ; http://n2t.net/addgene:61295 ; RRID:Addgene_61295)