pmIL-6 -231
(Plasmid #61287)

• Purpose: drives luciferase via mouse IL-6 promoter, truncated (-231)
• Depositing Lab: Gail Bishop
• Publication: Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

Backbone

• Vector backbone: pGL2 basic
• Backbone manufacturer: Promega
• Total vector size (bp): 6877
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Truncated IL-6 promoter
• Species: M. musculus (mouse)
• Mutation: Deletion of 1046 nucleotides from the 5' end of the IL-6 promoter (-231 from start site)
• Promoter: IL-6 promoter (truncated)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: EBV-rev
• 3′ sequencing primer: LucNRev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: See publication for details.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full sequence and map is available for wild-type vector pmIL-6 FL (Plasmid #61290). See publication for details of 5' sequence removed.