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Addgene

Xp75 CDD
(Plasmid #61283)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61283 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CS2+
  • Backbone size w/o insert (bp) 4097
  • Total vector size (bp) 5097
  • Vector type
    Mammalian Expression ; xenopus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p75 CDD
  • Alt name
    p75 neurotrophin receptor
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    750
  • Mutation
    lacks all but the first five amino acids of the putative cytoplasmic domain (contains aa 1-255)
  • Entrez Gene
    p75NTRa
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Xp75 CDD was a gift from Mark Bothwell & Lara Hutson (Addgene plasmid # 61283 ; http://n2t.net/addgene:61283 ; RRID:Addgene_61283)

  • For your References section:

    Expression and function of Xenopus laevis p75(NTR) suggest evolution of developmental regulatory mechanisms. Hutson LD, Bothwell M. J Neurobiol. 2001 Nov 5;49(2):79-98. 10.1002/neu.1067 PubMed 11598917
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