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Addgene

Zf Slit1b
(Plasmid #61280)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61280 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBSII SK(+)
  • Backbone manufacturer
    Stratagene
  • Total vector size (bp) 8100
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Slit1b
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    slit1b

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13pUC-Rev; T3
  • 3′ sequencing primer M13pUC-Fwd; T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

antisense probe: cut with SmaI and use T7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Zf Slit1b was a gift from Chi-Bin Chien & Lara Hutson (Addgene plasmid # 61280 ; http://n2t.net/addgene:61280 ; RRID:Addgene_61280)

  • For your References section:

    Two divergent slit1 genes in zebrafish. Hutson LD, Jurynec MJ, Yeo SY, Okamoto H, Chien CB. Dev Dyn. 2003 Nov;228(3):358-69. 10.1002/dvdy.10386 PubMed 14579375
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