pDest eGFP CUGBP1
(Plasmid #61276)

• Purpose: Mammalian expression of EGFP-tagged human CUGBP1
• Depositing Lab: Nicolas Charlet-Berguerand
• Publication: Charlet-Berguerand Plasmids (unpublished)

Backbone

• Vector backbone: pDEST
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CUG-BP
• Alt name: Elav-like family member 1
• Alt name: CELF1
• Species: H. sapiens (human)
• Entrez Gene: CELF1 (a.k.a. BRUNOL2, CUG-BP, CUGBP, CUGBP1, EDEN-BP, NAB50, NAPOR, hNab50)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: BGH-Rev

Resource Information

• Supplemental Documents:
  • pDest eGFP CUGBP1 genbank file
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry