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Addgene

pHIP_RNA2
(Plasmid #61260)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61260 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPD49.83
  • Backbone manufacturer
    Fire Lab Vector Kit
  • Backbone size w/o insert (bp) 3826
  • Total vector size (bp) 6435
  • Modifications to backbone
    HDV ribozyme added
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Orsay RNA2
  • Species
    Nematode virus
  • Insert Size (bp)
    2574
  • GenBank ID
    HM030971.2
  • Promoter heat shock promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pst1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer CGG AAC GTT GAG CTG GAC GGA
  • 3′ sequencing primer TTG GACTTA GAAGTCAGA GGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIP_RNA2 was a gift from David Wang (Addgene plasmid # 61260 ; http://n2t.net/addgene:61260 ; RRID:Addgene_61260)

  • For your References section:

    Engineering Recombinant Orsay Virus Directly in the Metazoan Host Caenorhabditis elegans. Jiang H, Franz CJ, Wang D. J Virol. 2014 Oct 15;88(20):11774-81. doi: 10.1128/JVI.01630-14. Epub 2014 Jul 30. 10.1128/JVI.01630-14 PubMed 25078701
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