Skip to main content
Addgene

pHIP_RNA1
(Plasmid #61259)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pPD49.83
  • Backbone manufacturer
    Fire Lab Vector Kit
  • Backbone size w/o insert (bp) 3826
  • Total vector size (bp) 7277
  • Modifications to backbone
    HDV ribozyme added
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Orsay RNA1
  • Species
    Nematode virus
  • Insert Size (bp)
    3421
  • GenBank ID
    HM030970.2
  • Promoter heat shock promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nsi1 (not destroyed)
  • 3′ cloning site Xma1 (not destroyed)
  • 5′ sequencing primer CGG AAC GTT GAG CTG GAC GGA
  • 3′ sequencing primer TTG GACTTA GAAGTCAGA GGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHIP_RNA1 was a gift from David Wang (Addgene plasmid # 61259 ; http://n2t.net/addgene:61259 ; RRID:Addgene_61259)

  • For your References section:

    Engineering Recombinant Orsay Virus Directly in the Metazoan Host Caenorhabditis elegans. Jiang H, Franz CJ, Wang D. J Virol. 2014 Oct 15;88(20):11774-81. doi: 10.1128/JVI.01630-14. Epub 2014 Jul 30. 10.1128/JVI.01630-14 PubMed 25078701
Related items:
Commonly requested with: