pJW1310
(Plasmid #61253)

• Purpose: Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion
• Depositing Lab: Jordan Ward
• Publication: Ward et al Genetics. 2014 Dec 9. pii: genetics.114.172361.

Backbone

• Vector backbone: pCR-Blunt II-TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3519
• Total vector size (bp): 4001
• Vector type: CRISPR ; Cloning template

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: U6 promoter
• Alt name: R07E5.16 promoter
• Species: C. elegans (nematode)
• Insert Size (bp): 482
• GenBank ID:

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: M13-rev
• 3′ sequencing primer: M13 forward

Resource Information

• Supplemental Documents:
  • pJW1310
• A portion of this plasmid was derived from a plasmid made by: U6 promoter was subcloned from pDD162 (AddGene plasmid #47549) and provided by the Goldstein lab.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry