pcDNA3-FLAG-hDIXDC1 S592A
(Plasmid
#61223)
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PurposeMammalian expression vector encoding long isoform of human DIXDC1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61223 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.2-DEST
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7711
- Total vector size (bp) 7400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman DIXDC1
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Alt nameCcd1
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SpeciesH. sapiens (human)
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Insert Size (bp)1900
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MutationChanged Serine 592 to Alanine
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GenBank IDNM_001037954.3
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Entrez GeneDIXDC1 (a.k.a. CCD1)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-FLAG-hDIXDC1 S592A was a gift from Reuben Shaw (Addgene plasmid # 61223 ; http://n2t.net/addgene:61223 ; RRID:Addgene_61223) -
For your References section:
An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Goodwin JM, Svensson RU, Lou HJ, Winslow MM, Turk BE, Shaw RJ. Mol Cell. 2014 Aug 7;55(3):436-50. doi: 10.1016/j.molcel.2014.06.021. Epub 2014 Jul 17. 10.1016/j.molcel.2014.06.021 PubMed 25042806