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Addgene

pLenti-PGK-Blast-FLAG-hDIXDC1 S592A
(Plasmid #61221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-PGK-BLAST
  • Backbone size w/o insert (bp) 9313
  • Total vector size (bp) 9000
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human DIXDC1
  • Alt name
    Ccd1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • Mutation
    Changed Serine 592 to Alanine
  • GenBank ID
    NM_001037954.3
  • Entrez Gene
    DIXDC1 (a.k.a. CCD1)
  • Promoter PGK
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-PGK-Blast-FLAG-hDIXDC1 S592A was a gift from Reuben Shaw (Addgene plasmid # 61221 ; http://n2t.net/addgene:61221 ; RRID:Addgene_61221)

  • For your References section:

    An AMPK-independent signaling pathway downstream of the LKB1 tumor suppressor controls Snail1 and metastatic potential. Goodwin JM, Svensson RU, Lou HJ, Winslow MM, Turk BE, Shaw RJ. Mol Cell. 2014 Aug 7;55(3):436-50. doi: 10.1016/j.molcel.2014.06.021. Epub 2014 Jul 17. 10.1016/j.molcel.2014.06.021 PubMed 25042806
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