6his R.3-AR GUSpET28a+/pLysS
(Plasmid #61157)

• Purpose: N-terminal His tagged round 3 aldehyde-resistant GUS expression vector
• Depositing Labs: Andrew Ellington, Ichiro Matsumura
• Publication: Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

Backbone

• Vector backbone: pET28a (+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BL21(DE3)
• Growth instructions: Contains pLysS plasmid (used for expressing low levels of T7 lysozyme). Maintain selection in kanamycin and chloramphenicol.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GUSAR
• Alt name: glutaraldehyde-resistant gusA
• Alt name: beta-glucuronidase
• Species: Escherichia coli
• Mutation: N66D, D151N, A219V, I396T, T480A, Q498R, D508E and K567R, compared to 6his-WT gusA-pET28a+
• Promoter: T7
• Tag / Fusion Protein:
  • 6x His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Matsumura lab strain number 342