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Addgene

C1A-C1A-EYFP
(Plasmid #61155)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61155 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat)
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1176
  • Mutation
    C1A from PKCG amino acids 26-89
  • GenBank ID
    24681
  • Entrez Gene
    Prkcg (a.k.a. PKC, PKCI, Prkc, Prkcc, RATPKCI)
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcorI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C1A-C1A-EYFP was a gift from Tobias Meyer (Addgene plasmid # 61155 ; http://n2t.net/addgene:61155 ; RRID:Addgene_61155)

  • For your References section:

    A polarized Ca(2+), diacylglycerol and STIM1 signalling system regulates directed cell migration. Tsai FC, Seki A, Yang HW, Hayer A, Carrasco S, Malmersjo S, Meyer T. Nat Cell Biol. 2014 Feb;16(2):133-44. doi: 10.1038/ncb2906. Epub 2014 Jan 26. 10.1038/ncb2906 PubMed 24463606
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