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Addgene

pB194
(Plasmid #61154)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS425Gal1
  • Backbone manufacturer
    Herren, B., and Pech, M. (1993) J. Recept. Res. 13, 725–738
  • Modifications to backbone
    pRS425Gal1 is pRS425 with a Gal1 promoter
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TFIIH complex serine/threonine-protein kinase subunit KIN28
  • Species
    S. cerevisiae (budding yeast)
  • GenBank ID
  • Entrez Gene
    KIN28 (a.k.a. YDL108W)
  • Promoter Gal1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer M13F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB194 was a gift from Charles Brenner (Addgene plasmid # 61154 ; http://n2t.net/addgene:61154 ; RRID:Addgene_61154)

  • For your References section:

    Adenosine monophosphoramidase activity of Hint and Hnt1 supports function of Kin28, Ccl1, and Tfb3. Bieganowski P, Garrison PN, Hodawadekar SC, Faye G, Barnes LD, Brenner C. J Biol Chem. 2002 Mar 29;277(13):10852-60. Epub 2002 Jan 22. 10.1074/jbc.M111480200 PubMed 11805111
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