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Addgene

Hs_MSI2_RBD
(Plasmid #61113)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61113 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22
  • Backbone size w/o insert (bp) 5464
  • Total vector size (bp) 6025
  • Modifications to backbone
    leader replaced with His tag and TEV protease site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MSI2 RNA binding domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    561
  • GenBank ID
    NM_138962.2
  • Entrez Gene
    MSI2 (a.k.a. MSI2H)
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hs_MSI2_RBD was a gift from Sean Ryder (Addgene plasmid # 61113 ; http://n2t.net/addgene:61113 ; RRID:Addgene_61113)
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