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Addgene

Dm_msi_RRM1
(Plasmid #61112)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61112 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5456
  • Total vector size (bp) 6011
  • Modifications to backbone
    leader replaced with His tag and TEV protease site
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Drosophila musashi RBD
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    555
  • Mutation
    stop codon after first RRM domain
  • GenBank ID
    NT_033777.3
  • Tag / Fusion Protein
    • 6xHis-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sac1 (not destroyed)
  • 3′ cloning site Sal1 (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dm_msi_RRM1 was a gift from Sean Ryder (Addgene plasmid # 61112 ; http://n2t.net/addgene:61112 ; RRID:Addgene_61112)
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