Skip to main content
Addgene

psc2_md1
(Plasmid #61093)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61093 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    psiCheck2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 6273
  • Total vector size (bp) 7231
  • Modifications to backbone
    HindIII site near Renilla mutated Methionine codon at beginning of firefly luciferase deleted Minimal, artificial upstream exon added before Mag genomic sequence
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mag genomic sequence
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    939
  • GenBank ID
    NM_010758
  • Entrez Gene
    Mag (a.k.a. Gma, siglec-4a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer custom
  • 3′ sequencing primer custom
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psc2_md1 was a gift from Sean Ryder (Addgene plasmid # 61093 ; http://n2t.net/addgene:61093 ; RRID:Addgene_61093)

  • For your References section:

    hnRNP A1 and secondary structure coordinate alternative splicing of Mag. Zearfoss NR, Johnson ES, Ryder SP. RNA. 2013 Jul;19(7):948-57. doi: 10.1261/rna.036780.112. Epub 2013 May 23. 10.1261/rna.036780.112 PubMed 23704325
Related items:
Commonly requested with: