MBP-mmA1
(Plasmid
#61090)
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Purposeexpression of fusion protein: MBP-hnRNP A1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61090 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHMTC
- Backbone size w/o insert (bp) 6700
- Total vector size (bp) 8700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehnRNP A1
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SpeciesM. musculus (mouse)
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Insert Size (bp)2000
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GenBank IDNM_010447.5
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Entrez GeneHnrnpa1 (a.k.a. HDP-1, Hdp, Hnrpa1, hnRNP A1, hnrnp-A1)
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Tag
/ Fusion Protein
- H6_MBP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site unknown (not destroyed)
- 5′ sequencing primer custom pMalSeq-1f
- 3′ sequencing primer custom pMalSeq-3r (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MBP-mmA1 was a gift from Sean Ryder (Addgene plasmid # 61090 ; http://n2t.net/addgene:61090 ; RRID:Addgene_61090) -
For your References section:
hnRNP A1 and secondary structure coordinate alternative splicing of Mag. Zearfoss NR, Johnson ES, Ryder SP. RNA. 2013 Jul;19(7):948-57. doi: 10.1261/rna.036780.112. Epub 2013 May 23. 10.1261/rna.036780.112 PubMed 23704325