pUC-H1-gRNA Notes
Addgene diagnostic digest
This gel depicts the NEB 1kb extend DNA ladder and a digestion of plasmid 61089 with HindIII-HF and EcoRI-HF.
We expected the following banding patterns:
HindIII-HF single digest: 1 band at 2981bp
EcoRI-HF single digest: 1 band at 2981bp
HindIII-HF / EcoRI-HF Double digest: 2 bands - ~350 and ~2600bp
Our restriction digests show the expected banding pattern.
