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Addgene

pDest27-mBIN1+13
(Plasmid #61087)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST27
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 8123
  • Total vector size (bp) 8472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse BIN1 with exon 13, excluding exon 7, 11, 14-17
  • Species
    M. musculus (mouse)
  • Promoter CMV
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest27-mBIN1+13 was a gift from Robin Shaw (Addgene plasmid # 61087 ; http://n2t.net/addgene:61087 ; RRID:Addgene_61087)

  • For your References section:

    Cardiac BIN1 folds T-tubule membrane, controlling ion flux and limiting arrhythmia. Hong T, Yang H, Zhang SS, Cho HC, Kalashnikova M, Sun B, Zhang H, Bhargava A, Grabe M, Olgin J, Gorelik J, Marban E, Jan LY, Shaw RM. Nat Med. 2014 Jun;20(6):624-32. doi: 10.1038/nm.3543. Epub 2014 May 18. 10.1038/nm.3543 PubMed 24836577
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