pJWV25
(Plasmid
#61045)
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Purpose(Empty Backbone) To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaA
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61045 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPPP1
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Vector typeBacterial Expression, Synthetic Biology
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Tag
/ Fusion Protein
- GFP+ (N terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsAmpicillin selection in E. coli, tetracycline selection in S. pneumoniae.
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJWV25 was a gift from Jan-Willem Veening (Addgene plasmid # 61045 ; http://n2t.net/addgene:61045 ; RRID:Addgene_61045) -
For your References section:
Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems. Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening JW. Mol Microbiol. 2009 Oct;74(2):395-408. doi: 10.1111/j.1365-2958.2009.06872.x. Epub 2009 Sep 8. 10.1111/j.1365-2958.2009.06872.x PubMed 19737355
