Cwc25-pRSETA
(Plasmid
#61043)
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PurposeEncodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 61043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSETA
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Backbone manufacturerInvitrogen Life Technologies
- Backbone size w/o insert (bp) 2900
- Total vector size (bp) 2900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCwc25
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)537
- Promoter T7
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Tag
/ Fusion Protein
- TEV site, Histidine Tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nde1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Cwc25-pRSETA was a gift from Nils Walter (Addgene plasmid # 61043 ; http://n2t.net/addgene:61043 ; RRID:Addgene_61043) -
For your References section:
Biased Brownian ratcheting leads to pre-mRNA remodeling and capture prior to first-step splicing. Krishnan R, Blanco MR, Kahlscheuer ML, Abelson J, Guthrie C, Walter NG. Nat Struct Mol Biol. 2013 Dec;20(12):1450-7. doi: 10.1038/nsmb.2704. Epub 2013 Nov 17. 10.1038/nsmb.2704 PubMed 24240612