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Addgene

pCRII HSPB12
(Plasmid #61037)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HSPB12
  • Species
    D. rerio (zebrafish)
  • GenBank ID
    CK689325
  • Entrez Gene
    hspb12 (a.k.a. fc17a04, wu:fc17a04)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

linearize with NotI, transcribe with SP6, probe length is 523.

Note: Addgene's quality control sequencing has found a few mismatches in the plasmid insert, but they should not affect its ability to be used as a probe.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRII HSPB12 was a gift from Lara Hutson (Addgene plasmid # 61037 ; http://n2t.net/addgene:61037 ; RRID:Addgene_61037)

  • For your References section:

    Developmental expression patterns of the zebrafish small heat shock proteins. Marvin M, O'Rourke D, Kurihara T, Juliano CE, Harrison KL, Hutson LD. Dev Dyn. 2008 Feb;237(2):454-63. 10.1002/dvdy.21414 PubMed 18161059
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