pCDNA3-V5-PTPN14-del-N
(Plasmid #61004)

• Purpose: Expresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tag
• Depositing Lab: Jianmin Zhang
• Publication: Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-700. doi: 10.1074/jbc.M113.534701. Epub 2014 Jul 14.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 8152
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTPN14 delete N
• Species: H. sapiens (human)
• Insert Size (bp): 2652
• Mutation: deleted amino acids 17-310
• Entrez Gene: PTPN14 (a.k.a. CATLPH, PEZ, PTP36, PTPD2)
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry