Skip to main content
Addgene

pCDNA3-V5-PTPN14-wild type
(Plasmid #61003)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61003 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 9061
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PTPN14
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3561
  • GenBank ID
    NM_005401
  • Entrez Gene
    PTPN14 (a.k.a. CATLPH, PEZ, PTP36, PTPD2)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-V5-PTPN14-wild type was a gift from Jianmin Zhang (Addgene plasmid # 61003 ; http://n2t.net/addgene:61003 ; RRID:Addgene_61003)

  • For your References section:

    PTPN14 forms a complex with Kibra and LATS1 proteins and negatively regulates the YAP oncogenic function. Wilson KE, Li YW, Yang N, Shen H, Orillion AR, Zhang J. J Biol Chem. 2014 Aug 22;289(34):23693-700. doi: 10.1074/jbc.M113.534701. Epub 2014 Jul 14. 10.1074/jbc.M113.534701 PubMed 25023289
Related items:
Commonly requested with: