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Addgene

pAMB_CAT-FspI
(Plasmid #61002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 61002 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAMB
  • Backbone manufacturer
    Ambion
  • Total vector size (bp) 3210
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    JM109
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chloramphenicol acetyltransferase
  • Insert Size (bp)
    657
  • Mutation
    FspI site introduced after T7 terminator
  • Promoter T7

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mutagenesis was done on control vector provided with the ActivePro™ In Vitro Translation Kit (Ambion)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAMB_CAT-FspI was a gift from Nils Walter (Addgene plasmid # 61002 ; http://n2t.net/addgene:61002 ; RRID:Addgene_61002)

  • For your References section:

    The Shine-Dalgarno sequence of riboswitch-regulated single mRNAs shows ligand-dependent accessibility bursts. Rinaldi AJ, Lund PE, Blanco MR, Walter NG. Nat Commun. 2016 Jan 19;7:8976. doi: 10.1038/ncomms9976. 10.1038/ncomms9976 PubMed 26781350
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