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Addgene

GOMO-GFP
(Plasmid #60975)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60975 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 2877
  • Total vector size (bp) 11696
  • Vector type
    Plasmodium gene targeting
  • Selectable markers
    hDHFR-yFCU

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    GFP
  • Species
    Synthetic
  • Insert Size (bp)
    726

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    714

Gene/Insert 3

  • Gene/Insert name
    hDHFR-yFCU
  • Species
    Synthetic
  • Insert Size (bp)
    1722

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GOMO-GFP was a gift from Olivier Silvie (Addgene plasmid # 60975 ; http://n2t.net/addgene:60975 ; RRID:Addgene_60975)
  • For your References section:

    A rapid and robust selection procedure for generating drug-selectable marker-free recombinant malaria parasites. Manzoni G, Briquet S, Risco-Castillo V, Gaultier C, Topcu S, Ivanescu ML, Franetich JF, Hoareau-Coudert B, Mazier D, Silvie O. Sci Rep. 2014 Apr 23;4:4760. doi: 10.1038/srep04760. 10.1038/srep04760 PubMed 24755823