GOMO-GFP
(Plasmid
#60975)
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PurposeRapid selection of recombinant marker-free fluorescent rodent malaria parasites
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 60975 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Backbone size w/o insert (bp) 2877
- Total vector size (bp) 11696
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Vector typePlasmodium gene targeting
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Selectable markershDHFR-yFCU
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameGFP
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SpeciesSynthetic
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Insert Size (bp)726
Gene/Insert 2
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Gene/Insert namemCherry
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SpeciesSynthetic
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Insert Size (bp)714
Gene/Insert 3
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Gene/Insert namehDHFR-yFCU
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SpeciesSynthetic
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Insert Size (bp)1722
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GOMO-GFP was a gift from Olivier Silvie (Addgene plasmid # 60975 ; http://n2t.net/addgene:60975 ; RRID:Addgene_60975) -
For your References section:
A rapid and robust selection procedure for generating drug-selectable marker-free recombinant malaria parasites. Manzoni G, Briquet S, Risco-Castillo V, Gaultier C, Topcu S, Ivanescu ML, Franetich JF, Hoareau-Coudert B, Mazier D, Silvie O. Sci Rep. 2014 Apr 23;4:4760. doi: 10.1038/srep04760. 10.1038/srep04760 PubMed 24755823