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Addgene

pT7-gRNA:kit-1
(Plasmid #60969)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60969 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    DR274
  • Backbone size w/o insert (bp) 2147
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA
  • Alt name
    Kit
  • Alt name
    H
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    20
  • Entrez Gene
    Kit

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Targets an integrated 7,098-bp endogenous retroviral element (ERV) within the first intron of the Kit gene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-gRNA:kit-1 was a gift from Tomoji Mashimo (Addgene plasmid # 60969 ; http://n2t.net/addgene:60969 ; RRID:Addgene_60969)

  • For your References section:

    Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Yoshimi K, Kaneko T, Voigt B, Mashimo T. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240. 10.1038/ncomms5240 PubMed 24967838
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