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Addgene

pT7-gRNA:Tyr (albino)
(Plasmid #60966)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60966 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    DR274
  • Backbone size w/o insert (bp) 2147
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tyrosinase gRNA
  • Alt name
    albino
  • Alt name
    c
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    20
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (not destroyed)
  • 3′ cloning site BsaI (not destroyed)
  • 5′ sequencing primer M13 forward
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Targets a mutation 896G>A in exon 2 of the Tyr gene resulting in an Arg299His missense mutation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7-gRNA:Tyr (albino) was a gift from Tomoji Mashimo (Addgene plasmid # 60966 ; http://n2t.net/addgene:60966 ; RRID:Addgene_60966)

  • For your References section:

    Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Yoshimi K, Kaneko T, Voigt B, Mashimo T. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240. 10.1038/ncomms5240 PubMed 24967838
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