pEP-EGFPin
(Plasmid #60961)

• Purpose: PCR template for the insertion of eGFP by en passant (two-step Red-mediated) mutagenesis
• Depositing Lab: Nikolaus Osterrieder
• Publication: Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Backbone

• Vector backbone: pEGFP N1
• Total vector size (bp): 4637

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: eGFP-Kana_I-SceI

Resource Information

• Supplemental Documents:
  • pEP-EGFPin - GenBank file
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry