pBAD-I-SceI
(Plasmid #60960)

• Purpose: Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis
• Depositing Lab: Nikolaus Osterrieder
• Publication: Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Backbone

• Vector backbone: pBAD24
• Total vector size (bp): 5238

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: I-SceI restriction enzyme

Resource Information

• Supplemental Documents:
  • pBAD-I-SceI - GenBank file
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry