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Addgene

pSwith-tdTomato
(Plasmid #60953)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60953 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXs
  • Backbone manufacturer
    GeneArt
  • Backbone size w/o insert (bp) 2450
  • Vector type
    Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tdTomato
  • Insert Size (bp)
    1400

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site SpeI (unknown if destroyed)
  • 5′ sequencing primer M13-F20
  • 3′ sequencing primer M13-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSwith-tdTomato was a gift from Liangxue Lai (Addgene plasmid # 60953 ; http://n2t.net/addgene:60953 ; RRID:Addgene_60953)

  • For your References section:

    Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing. Li X, Yang Y, Bu L, Guo X, Tang C, Song J, Fan N, Zhao B, Ouyang Z, Liu Z, Zhao Y, Yi X, Quan L, Liu S, Yang Z, Ouyang H, Chen YE, Wang Z, Lai L. Cell Res. 2014 Apr;24(4):501-4. doi: 10.1038/cr.2014.15. Epub 2014 Feb 7. 10.1038/cr.2014.15 PubMed 24503648
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