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Addgene

pROSA26-Donor
(Plasmid #60952)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60952 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFlexibleDT
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Gene targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Porcine ROSA26 homologous ARM
  • Species
    Pig
  • Insert Size (bp)
    9300
  • Tag / Fusion Protein
    • Neo EGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The note that the discrepancies between the full and QC sequence are not of functional concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pROSA26-Donor was a gift from Liangxue Lai (Addgene plasmid # 60952 ; http://n2t.net/addgene:60952 ; RRID:Addgene_60952)

  • For your References section:

    Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing. Li X, Yang Y, Bu L, Guo X, Tang C, Song J, Fan N, Zhao B, Ouyang Z, Liu Z, Zhao Y, Yi X, Quan L, Liu S, Yang Z, Ouyang H, Chen YE, Wang Z, Lai L. Cell Res. 2014 Apr;24(4):501-4. doi: 10.1038/cr.2014.15. Epub 2014 Feb 7. 10.1038/cr.2014.15 PubMed 24503648
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