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PurposeExpresses Tet3 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 60940 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA-flag
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTen-Eleven Translocation 3
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SpeciesM. musculus (mouse)
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Insert Size (bp)5763
- Promoter CMV
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Tag
/ Fusion Protein
- flag (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-Flag-Tet3 was a gift from Yi Zhang (Addgene plasmid # 60940 ; http://n2t.net/addgene:60940 ; RRID:Addgene_60940) -
For your References section:
Regulation of TET protein stability by calpains. Wang Y, Zhang Y. Cell Rep. 2014 Jan 30;6(2):278-84. doi: 10.1016/j.celrep.2013.12.031. Epub 2014 Jan 9. 10.1016/j.celrep.2013.12.031 PubMed 24412366
