pcDNA3-Tet2
(Plasmid #60939)

• Purpose: Expresses Tet2 in mammalian cells
• Depositing Lab: Yi Zhang
• Publication: Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi: 10.1016/j.celrep.2013.12.031. Epub 2014 Jan 9.

Backbone

• Vector backbone: pcDNA3*
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ten-Eleven Translocation 2
• Alt name: tet2
• Species: M. musculus (mouse)
• Insert Size (bp): 5763
• Entrez Gene: Tet2 (a.k.a. Ayu17-449, E130014J05Rik, mKIAA1546)
• Promoter: CMV
• Tag / Fusion Protein:
  • flag (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins