pPL5618_pUG_MtxR
(Plasmid #60929)

• Purpose: PCR template vector for generating methotrexate resistance cassette.
• Depositing Lab: Robert Piper
• Publication: MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.3069.

Backbone

• Vector backbone: pUG27
• Vector type: LoxP flanked deletion cassette template

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dihydrofolate reductase
• Alt name: DHFR
• Species: H. sapiens (human)
• Insert Size (bp): 561
• Mutation: L22F F31S
• Entrez Gene: DHFR (a.k.a. DHFR1, DHFRP1, DYR)
• Promoter: TEF1

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: cctcgctgcagacctgcgagcagg
• 3′ sequencing primer: gggcagatgatgtcgaggcg

Resource Information

• A portion of this plasmid was derived from a plasmid made by: They were gene synthesized using a commercial service, which made them according to our design.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dihydrofolate reductase gene from Homo sapiens carrying L22F and F31S mutations (DHFR*); codon optimized for expression in Saccharomyces cerevisiae. It also contains an additional F180L mutation that does not impact protein function.