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pPL5617_pUG_PuroR
(Plasmid #60928)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60928 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUG27
  • Vector type
    LoxP flanked deletion cassette template
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    puromycin N-Acetyltransferase
  • Alt name
    pac
  • Insert Size (bp)
    597
  • Promoter TEF1

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ggttcttctttcatatacttcc
  • 3′ sequencing primer gggcagatgatgtcgaggcg
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    They were gene synthesized using a commercial service, which made them according to our design.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Puromycin resistance gene from Streptomyces alboniger; codon optimized for expression in Saccharomyces cerevisiae

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPL5617_pUG_PuroR was a gift from Robert Piper (Addgene plasmid # 60928 ; http://n2t.net/addgene:60928 ; RRID:Addgene_60928)

  • For your References section:

    Puromycin and Methotrexate Resistance Cassettes and Optimized cre-recombinase Expression Plasmids for use in Yeast. MacDonald C, Piper RC. Yeast. 2015 Feb 16. doi: 10.1002/yea.3069. 10.1002/yea.3069 PubMed 25688547
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