Skip to main content
Addgene

pcDNA3-Flag-Tpr-Si
(Plasmid #60918)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60918 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TPR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7049
  • Mutation
    Silent point mutations in siRNA target region; E1239Q
  • Entrez Gene
    TPR (a.k.a. MRT79)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not-I (unknown if destroyed)
  • 3′ cloning site Apa-I (unknown if destroyed)
  • 5′ sequencing primer 5' taa caa ctc cgc ccc att ga 3’
  • 3′ sequencing primer 5’ atg caa ttt cct cat ttt at 3’
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Tpr insert in this plasmid contains a E1239Q mutation compared to GenBank reference sequence CAA47021.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-Flag-Tpr-Si was a gift from Vinay Nandicoori (Addgene plasmid # 60918 ; http://n2t.net/addgene:60918 ; RRID:Addgene_60918)

  • For your References section:

    Localization of nucleoporin Tpr to the nuclear pore complex is essential for Tpr mediated regulation of the export of unspliced RNA. Rajanala K, Nandicoori VK. PLoS One. 2012;7(1):e29921. doi: 10.1371/journal.pone.0029921. Epub 2012 Jan 13. 10.1371/journal.pone.0029921 PubMed 22253824
Related items:
Commonly requested with: