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pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE Citations (25)

Originally described in: A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.
Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. PubMed Journal

Articles Citing pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE

Articles
Specific Reactivation of Latent HIV-1 by dCas9-SunTag-VP64-mediated Guide RNA Targeting the HIV-1 Promoter. Ji H, Jiang Z, Lu P, Ma L, Li C, Pan H, Fu Z, Qu X, Wang P, Deng J, Yang X, Wang J, Zhu H. Mol Ther. 2016 Mar;24(3):508-21. doi: 10.1038/mt.2016.7. Epub 2016 Jan 18. PubMed
Versatile protein tagging in cells with split fluorescent protein. Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B. Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046. PubMed

Associated Plasmids
Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells. Pichon X, Bastide A, Safieddine A, Chouaib R, Samacoits A, Basyuk E, Peter M, Mueller F, Bertrand E. J Cell Biol. 2016 Sep 12;214(6):769-81. doi: 10.1083/jcb.201605024. Epub 2016 Sep 5. PubMed
Specific Expression of Interferon-gamma Induced by Synergistic Activation Mediator-Derived Systems Activates Innate Immunity and Inhibits Tumorigenesis. Liu S, Yu X, Wang Q, Liu Z, Xiao Q, Hou P, Hu Y, Hou W, Yang Z, Guo D, Chen S. J Microbiol Biotechnol. 2017 Oct 28;27(10):1855-1866. doi: 10.4014/jmb.1705.05081. PubMed
Multiplexed sgRNA Expression Allows Versatile Single Nonrepetitive DNA Labeling and Endogenous Gene Regulation. Shao S, Chang L, Sun Y, Hou Y, Fan X, Sun Y. ACS Synth Biol. 2017 Sep 7. doi: 10.1021/acssynbio.7b00268. PubMed

Associated Plasmids
TUBB3 overexpression has a negligible effect on the sensitivity to taxol in cultured cell lines. Tame MA, Manjon AG, Belokhvostova D, Raaijmakers JA, Medema RH. Oncotarget. 2017 May 10;8(42):71536-71547. doi: 10.18632/oncotarget.17740. eCollection 2017 Sep 22. PubMed
(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes. Neguembor MV, Sebastian-Perez R, Aulicino F, Gomez-Garcia PA, Cosma MP, Lakadamyali M. Nucleic Acids Res. 2018 Mar 16;46(5):e30. doi: 10.1093/nar/gkx1271. PubMed
Comparison and optimization of CRISPR/dCas9/gRNA genome-labeling systems for live cell imaging. Hong Y, Lu G, Duan J, Liu W, Zhang Y. Genome Biol. 2018 Mar 22;19(1):39. doi: 10.1186/s13059-018-1413-5. PubMed
Co-optation of Tandem DNA Repeats for the Maintenance of Mesenchymal Identity. Balestrieri C, Alfarano G, Milan M, Tosi V, Prosperini E, Nicoli P, Palamidessi A, Scita G, Diaferia GR, Natoli G. Cell. 2018 May 17;173(5):1150-1164.e14. doi: 10.1016/j.cell.2018.03.081. Epub 2018 Apr 26. PubMed
Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag. Chen B, Zou W, Xu H, Liang Y, Huang B. Nat Commun. 2018 Nov 29;9(1):5065. doi: 10.1038/s41467-018-07498-y. PubMed

Associated Plasmids
Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen NS, Haataja M, Brangwynne CP. Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057. PubMed

Associated Plasmids
NATF (Native And Tissue-Specific Fluorescence): A Strategy for Bright, Tissue-Specific GFP Labeling of Native Proteins in Caenorhabditis elegans. He S, Cuentas-Condori A, Miller DM. Genetics. 2019 Apr 5. pii: genetics.119.302063. doi: 10.1534/genetics.119.302063. PubMed

Associated Plasmids
Engineering of Effector Domains for Targeted DNA Methylation with Reduced Off-Target Effects. Hofacker D, Broche J, Laistner L, Adam S, Bashtrykov P, Jeltsch A. Int J Mol Sci. 2020 Jan 13;21(2). pii: ijms21020502. doi: 10.3390/ijms21020502. PubMed

Associated Plasmids
Visualizing the translation and packaging of HIV-1 full-length RNA. Chen J, Liu Y, Wu B, Nikolaitchik OA, Mohan PR, Chen J, Pathak VK, Hu WS. Proc Natl Acad Sci U S A. 2020 Mar 17;117(11):6145-6155. doi: 10.1073/pnas.1917590117. Epub 2020 Mar 4. PubMed
Histone methyltransferase activity programs nuclear peripheral genome positioning. See K, Kiseleva AA, Smith CL, Liu F, Li J, Poleshko A, Epstein JA. Dev Biol. 2020 Oct 1;466(1-2):90-98. doi: 10.1016/j.ydbio.2020.07.010. Epub 2020 Jul 24. PubMed
Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore. Chaudhary N, Nho SH, Cho H, Gantumur N, Ra JS, Myung K, Kim H. Genome Res. 2020 Sep;30(9):1306-1316. doi: 10.1101/gr.260018.119. Epub 2020 Sep 4. PubMed

Associated Plasmids
CRISPR-Sunspot: Imaging of endogenous low-abundance RNA at the single-molecule level in live cells. Sun NH, Chen DY, Ye LP, Sheng G, Gong JJ, Chen BH, Lu YM, Han F. Theranostics. 2020 Sep 2;10(24):10993-11012. doi: 10.7150/thno.43094. eCollection 2020. PubMed
Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells. Chang L, Li M, Shao S, Li C, Ai S, Xue B, Hou Y, Zhang Y, Li R, Fan X, He A, Li C, Sun Y. Protein Cell. 2022 Apr;13(4):258-280. doi: 10.1007/s13238-020-00794-8. Epub 2020 Nov 5. PubMed
MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo. Zhang X, Lv S, Luo Z, Hu Y, Peng X, Lv J, Zhao S, Feng J, Huang G, Wan QL, Liu J, Huang H, Luan B, Wang D, Zhao X, Lin Y, Zhou Q, Zhang ZN, Rong Z. Nucleic Acids Res. 2021 Mar 22. pii: 6179929. doi: 10.1093/nar/gkab174. PubMed

Associated Plasmids
Transcriptional Activation of Heterochromatin by Recruitment of dCas9 Activators. Frank L, Weinmann R, Erdel F, Trojanowski J, Rippe K. Methods Mol Biol. 2021;2351:307-320. doi: 10.1007/978-1-0716-1597-3_17. PubMed
Unexpected gene activation following CRISPR-Cas9-mediated genome editing. Manjon AG, Linder S, Teunissen H, Friskes A, Zwart W, de Wit E, Medema RH. EMBO Rep. 2022 Feb 3;23(2):e53902. doi: 10.15252/embr.202153902. Epub 2021 Dec 20. PubMed
Super resolution microscopy reveals how elongating RNA polymerase II and nascent RNA interact with nucleosome clutches. Castells-Garcia A, Ed-Daoui I, Gonzalez-Almela E, Vicario C, Ottestrom J, Lakadamyali M, Neguembor MV, Cosma MP. Nucleic Acids Res. 2022 Jan 11;50(1):175-190. doi: 10.1093/nar/gkab1215. PubMed
Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system. Chen M, Sui T, Yang L, Qian Y, Liu Z, Liu Y, Wang G, Lai L, Li Z. Biosens Bioelectron. 2022 May 15;204:114074. doi: 10.1016/j.bios.2022.114074. Epub 2022 Feb 4. PubMed
Viscosity-aided electromechanical poration of cells for transfecting molecules. Huang W, Sakuma S, Tottori N, Sugano SS, Yamanishi Y. Lab Chip. 2022 Nov 8;22(22):4276-4291. doi: 10.1039/d2lc00628f. PubMed
Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells. Peng Q, Huang Z, Sun K, Liu Y, Yoon CW, Harrison RES, Schmitt DL, Zhu L, Wu Y, Tasan I, Zhao H, Zhang J, Zhong S, Chien S, Wang Y. Nat Commun. 2022 Dec 24;13(1):7933. doi: 10.1038/s41467-022-35504-x. PubMed

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