pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE Citations (26)
Originally described in: A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. PubMed Journal
Articles Citing pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE
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| CRISPR/dCas9-mediated Transcriptional Inhibition Ameliorates the Epigenetic Dysregulation at D4Z4 and Represses DUX4-fl in FSH Muscular Dystrophy. Himeda CL, Jones TI, Jones PL. Mol Ther. 2015 Nov 3. doi: 10.1038/mt.2015.200. PubMed |
| Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells. Pichon X, Bastide A, Safieddine A, Chouaib R, Samacoits A, Basyuk E, Peter M, Mueller F, Bertrand E. J Cell Biol. 2016 Sep 12;214(6):769-81. doi: 10.1083/jcb.201605024. Epub 2016 Sep 5. PubMed |
| The methyltransferase SETDB1 regulates a large neuron-specific topological chromatin domain. Jiang Y, Loh YE, Rajarajan P, Hirayama T, Liao W, Kassim BS, Javidfar B, Hartley BJ, Kleofas L, Park RB, Labonte B, Ho SM, Chandrasekaran S, Do C, Ramirez BR, Peter CJ, C W JT, Safaie BM, Morishita H, Roussos P, Nestler EJ, Schaefer A, Tycko B, Brennand KJ, Yagi T, Shen L, Akbarian S. Nat Genet. 2017 Aug;49(8):1239-1250. doi: 10.1038/ng.3906. Epub 2017 Jul 3. PubMed |
Multiplexed sgRNA Expression Allows Versatile Single Nonrepetitive DNA Labeling and Endogenous Gene Regulation.
Shao S, Chang L, Sun Y, Hou Y, Fan X, Sun Y.
ACS Synth Biol. 2017 Sep 7. doi: 10.1021/acssynbio.7b00268.
PubMed
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| (Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes. Neguembor MV, Sebastian-Perez R, Aulicino F, Gomez-Garcia PA, Cosma MP, Lakadamyali M. Nucleic Acids Res. 2018 Mar 16;46(5):e30. doi: 10.1093/nar/gkx1271. PubMed |
| A hybridization-chain-reaction-based method for amplifying immunosignals. Lin R, Feng Q, Li P, Zhou P, Wang R, Liu Z, Wang Z, Qi X, Tang N, Shao F, Luo M. Nat Methods. 2018 Apr;15(4):275-278. doi: 10.1038/nmeth.4611. Epub 2018 Feb 26. PubMed |
| Dynamics of sister chromatid resolution during cell cycle progression. Stanyte R, Nuebler J, Blaukopf C, Hoefler R, Stocsits R, Peters JM, Gerlich DW. J Cell Biol. 2018 Jun 4;217(6):1985-2004. doi: 10.1083/jcb.201801157. Epub 2018 Apr 25. PubMed |
Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome.
Shin Y, Chang YC, Lee DSW, Berry J, Sanders DW, Ronceray P, Wingreen NS, Haataja M, Brangwynne CP.
Cell. 2018 Nov 29;175(6):1481-1491.e13. doi: 10.1016/j.cell.2018.10.057.
PubMed
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| Translation imaging of single mRNAs in established cell lines and primary cultured neurons. Latallo MJ, Livingston NM, Wu B. Methods. 2019 Jun 1;162-163:12-22. doi: 10.1016/j.ymeth.2019.03.021. Epub 2019 Mar 21. PubMed |
| Translational regulation of protrusion-localized RNAs involves silencing and clustering after transport. Moissoglu K, Yasuda K, Wang T, Chrisafis G, Mili S. Elife. 2019 Jul 10;8. pii: 44752. doi: 10.7554/eLife.44752. PubMed |
Engineering of Effector Domains for Targeted DNA Methylation with Reduced Off-Target Effects.
Hofacker D, Broche J, Laistner L, Adam S, Bashtrykov P, Jeltsch A.
Int J Mol Sci. 2020 Jan 13;21(2). pii: ijms21020502. doi: 10.3390/ijms21020502.
PubMed
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| Histone methyltransferase activity programs nuclear peripheral genome positioning. See K, Kiseleva AA, Smith CL, Liu F, Li J, Poleshko A, Epstein JA. Dev Biol. 2020 Oct 1;466(1-2):90-98. doi: 10.1016/j.ydbio.2020.07.010. Epub 2020 Jul 24. PubMed |
Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore.
Chaudhary N, Nho SH, Cho H, Gantumur N, Ra JS, Myung K, Kim H.
Genome Res. 2020 Sep;30(9):1306-1316. doi: 10.1101/gr.260018.119. Epub 2020 Sep 4.
PubMed
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| Illuminating single genomic loci in live cells by reducing nuclear background fluorescence. Lu S, Wang D, Hou Y, Guo D, Deng Y, Zhang XE. Sci China Life Sci. 2021 May;64(5):667-677. doi: 10.1007/s11427-020-1794-2. Epub 2020 Oct 26. PubMed |
| Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells. Chang L, Li M, Shao S, Li C, Ai S, Xue B, Hou Y, Zhang Y, Li R, Fan X, He A, Li C, Sun Y. Protein Cell. 2022 Apr;13(4):258-280. doi: 10.1007/s13238-020-00794-8. Epub 2020 Nov 5. PubMed |
| Dynamics of hunchback translation in real-time and at single-mRNA resolution in the Drosophila embryo. Vinter DJ, Hoppe C, Minchington TG, Sutcliffe C, Ashe HL. Development. 2021 Sep 15;148(18). pii: dev.196121. doi: 10.1242/dev.196121. Epub 2021 Apr 15. PubMed |
| Different translation dynamics of beta- and gamma-actin regulates cell migration. Vedula P, Kurosaka S, MacTaggart B, Ni Q, Papoian G, Jiang Y, Dong DW, Kashina A. Elife. 2021 Jun 24;10. pii: 68712. doi: 10.7554/eLife.68712. PubMed |
| Translational regulation in the brain by TDP-43 phase separation. Gao J, Wang L, Ren X, Dunn JR, Peters A, Miyagi M, Fujioka H, Zhao F, Askwith C, Liang J, Wang X. J Cell Biol. 2021 Oct 4;220(10):e202101019. doi: 10.1083/jcb.202101019. Epub 2021 Aug 24. PubMed |
| The kinesin KIF1C transports APC-dependent mRNAs to cell protrusions. Pichon X, Moissoglu K, Coleno E, Wang T, Imbert A, Robert MC, Peter M, Chouaib R, Walter T, Mueller F, Zibara K, Bertrand E, Mili S. RNA. 2021 Dec;27(12):1528-1544. doi: 10.1261/rna.078576.120. Epub 2021 Sep 7. PubMed |
| Super resolution microscopy reveals how elongating RNA polymerase II and nascent RNA interact with nucleosome clutches. Castells-Garcia A, Ed-Daoui I, Gonzalez-Almela E, Vicario C, Ottestrom J, Lakadamyali M, Neguembor MV, Cosma MP. Nucleic Acids Res. 2022 Jan 11;50(1):175-190. doi: 10.1093/nar/gkab1215. PubMed |
| CRISPR Cas13-Based Tools to Track and Manipulate Endogenous Telomeric Repeat-Containing RNAs in Live Cells. Xu M, Chigumira T, Chen Z, Tones J, Zhao R, Dahl KN, Chenoweth DM, Zhang H. Front Mol Biosci. 2022 Jan 31;8:785160. doi: 10.3389/fmolb.2021.785160. eCollection 2021. PubMed |
| Optogenetic Control of Background Fluorescence Reduction for CRISPR-Based Genome Imaging. Hou Y, Wang D, Lu S, Guo D, Li M, Cui M, Zhang XE. Anal Chem. 2022 Jun 21;94(24):8724-8731. doi: 10.1021/acs.analchem.2c01113. Epub 2022 Jun 6. PubMed |
| Engineering inducible biomolecular assemblies for genome imaging and manipulation in living cells. Peng Q, Huang Z, Sun K, Liu Y, Yoon CW, Harrison RES, Schmitt DL, Zhu L, Wu Y, Tasan I, Zhao H, Zhang J, Zhong S, Chien S, Wang Y. Nat Commun. 2022 Dec 24;13(1):7933. doi: 10.1038/s41467-022-35504-x. PubMed |
| mRNA Location and Translation Rate Determine Protein Targeting to Dual Destinations. Gasparski AN, Moissoglu K, Pallikkuth S, Meydan S, Guydosh NR, Mili S. bioRxiv. 2023 Apr 26:2023.04.24.538105. doi: 10.1101/2023.04.24.538105. Preprint. PubMed |
| Mitotic clustering of pulverized chromosomes from micronuclei. Lin YF, Hu Q, Mazzagatti A, Valle-Inclan JE, Maurais EG, Dahiya R, Guyer A, Sanders JT, Engel JL, Nguyen G, Bronder D, Bakhoum SF, Cortes-Ciriano I, Ly P. Nature. 2023 Jun;618(7967):1041-1048. doi: 10.1038/s41586-023-05974-0. Epub 2023 May 10. PubMed |
Modular dual-color BiAD sensors for locus-specific readout of epigenome modifications in single cells.
Kohler AR, Hausser J, Harsch A, Bernhardt S, Haussermann L, Brenner LM, Lungu C, Olayioye MA, Bashtrykov P, Jeltsch A.
Cell Rep Methods. 2024 Mar 26:100739. doi: 10.1016/j.crmeth.2024.100739.
PubMed
Associated Plasmids |
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