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pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP Citations (37)

Originally described in: A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.
Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039. PubMed Journal

Articles Citing pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

Articles
CRISPR/dCas9-mediated Transcriptional Inhibition Ameliorates the Epigenetic Dysregulation at D4Z4 and Represses DUX4-fl in FSH Muscular Dystrophy. Himeda CL, Jones TI, Jones PL. Mol Ther. 2015 Nov 3. doi: 10.1038/mt.2015.200. PubMed
CRISPR Technology for Genome Activation and Repression in Mammalian Cells. Du D, Qi LS. Cold Spring Harb Protoc. 2016 Jan 4;2016(1):pdb.prot090175. doi: 10.1101/pdb.prot090175. PubMed
Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed
Comparison of Cas9 activators in multiple species. Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871. PubMed

Associated Plasmids
Targeted HIV-1 Latency Reversal Using CRISPR/Cas9-Derived Transcriptional Activator Systems. Bialek JK, Dunay GA, Voges M, Schafer C, Spohn M, Stucka R, Hauber J, Lange UC. PLoS One. 2016 Jun 24;11(6):e0158294. doi: 10.1371/journal.pone.0158294. eCollection 2016. PubMed
The methyltransferase SETDB1 regulates a large neuron-specific topological chromatin domain. Jiang Y, Loh YE, Rajarajan P, Hirayama T, Liao W, Kassim BS, Javidfar B, Hartley BJ, Kleofas L, Park RB, Labonte B, Ho SM, Chandrasekaran S, Do C, Ramirez BR, Peter CJ, C W JT, Safaie BM, Morishita H, Roussos P, Nestler EJ, Schaefer A, Tycko B, Brennand KJ, Yagi T, Shen L, Akbarian S. Nat Genet. 2017 Aug;49(8):1239-1250. doi: 10.1038/ng.3906. Epub 2017 Jul 3. PubMed
CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation. Nihongaki Y, Furuhata Y, Otabe T, Hasegawa S, Yoshimoto K, Sato M. Nat Methods. 2017 Oct;14(10):963-966. doi: 10.1038/nmeth.4430. Epub 2017 Sep 11. PubMed
DNA epigenome editing using CRISPR-Cas SunTag-directed DNMT3A. Huang YH, Su J, Lei Y, Brunetti L, Gundry MC, Zhang X, Jeong M, Li W, Goodell MA. Genome Biol. 2017 Sep 18;18(1):176. doi: 10.1186/s13059-017-1306-z. PubMed

Associated Plasmids
Combinatorial genetics in liver repopulation and carcinogenesis with a novel in vivo CRISPR activation platform. Wangensteen KJ, Wang YJ, Dou Z, Wang AW, Mosleh-Shirazi E, Horlbeck MA, Gilbert LA, Weissman JS, Berger SL, Kaestner KH. Hepatology. 2017 Nov 1. doi: 10.1002/hep.29626. PubMed
(Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes. Neguembor MV, Sebastian-Perez R, Aulicino F, Gomez-Garcia PA, Cosma MP, Lakadamyali M. Nucleic Acids Res. 2018 Mar 16;46(5):e30. doi: 10.1093/nar/gkx1271. PubMed
Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain. Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo HY, Zhao JM, Segal DJ, Jacobsen SE. Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 10.1073/pnas.1716945115. Epub 2018 Feb 14. PubMed

Associated Plasmids
Dynamics of sister chromatid resolution during cell cycle progression. Stanyte R, Nuebler J, Blaukopf C, Hoefler R, Stocsits R, Peters JM, Gerlich DW. J Cell Biol. 2018 Jun 4;217(6):1985-2004. doi: 10.1083/jcb.201801157. Epub 2018 Apr 25. PubMed
A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. Pflueger C, Tan D, Swain T, Nguyen TV, Pflueger J, Nefzger C, Polo JM, Ford E, Lister R. Genome Res. 2018 Jun 15. pii: gr.233049.117. doi: 10.1101/gr.233049.117. PubMed

Associated Plasmids
Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. PubMed

Associated Plasmids
The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Heurtier V, Owens N, Gonzalez I, Mueller F, Proux C, Mornico D, Clerc P, Dubois A, Navarro P. Nat Commun. 2019 Mar 7;10(1):1109. doi: 10.1038/s41467-019-09041-z. PubMed

Associated Plasmids
Transposable elements drive widespread expression of oncogenes in human cancers. Jang HS, Shah NM, Du AY, Dailey ZZ, Pehrsson EC, Godoy PM, Zhang D, Li D, Xing X, Kim S, O'Donnell D, Gordon JI, Wang T. Nat Genet. 2019 Apr;51(4):611-617. doi: 10.1038/s41588-019-0373-3. Epub 2019 Mar 29. PubMed
Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development. Genga RMJ, Kernfeld EM, Parsi KM, Parsons TJ, Ziller MJ, Maehr R. Cell Rep. 2019 Apr 16;27(3):708-718.e10. doi: 10.1016/j.celrep.2019.03.076. PubMed

Associated Plasmids
Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors. Tran NT, Bashir S, Li X, Rossius J, Chu VT, Rajewsky K, Kuhn R. Front Genet. 2019 Apr 30;10:365. doi: 10.3389/fgene.2019.00365. eCollection 2019. PubMed
In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Peter CJ, Saito A, Hasegawa Y, Tanaka Y, Nagpal M, Perez G, Alway E, Espeso-Gil S, Fayyad T, Ratner C, Dincer A, Gupta A, Devi L, Pappas JG, Lalonde FM, Butman JA, Han JC, Akbarian S, Kamiya A. Nat Commun. 2019 Sep 11;10(1):4112. doi: 10.1038/s41467-019-12013-y. PubMed
A curative combination cancer therapy achieves high fractional cell killing through low cross-resistance and drug additivity. Palmer AC, Chidley C, Sorger PK. Elife. 2019 Nov 19;8. pii: 50036. doi: 10.7554/eLife.50036. PubMed
Engineering of Effector Domains for Targeted DNA Methylation with Reduced Off-Target Effects. Hofacker D, Broche J, Laistner L, Adam S, Bashtrykov P, Jeltsch A. Int J Mol Sci. 2020 Jan 13;21(2). pii: ijms21020502. doi: 10.3390/ijms21020502. PubMed

Associated Plasmids
Robustness of Catalytically Dead Cas9 Activators in Human Pluripotent and Mesenchymal Stem Cells. Petazzi P, Torres-Ruiz R, Fidanza A, Roca-Ho H, Gutierrez-Aguera F, Castano J, Rodriguez-Perales S, Diaz de la Guardia R, Lopez-Millan B, Bigas A, Forrester LM, Bueno C, Menendez P. Mol Ther Nucleic Acids. 2020 Jun 5;20:196-204. doi: 10.1016/j.omtn.2020.02.009. Epub 2020 Feb 27. PubMed
Transcription factor competition at the gamma-globin promoters controls hemoglobin switching. Liu N, Xu S, Yao Q, Zhu Q, Kai Y, Hsu JY, Sakon P, Pinello L, Yuan GC, Bauer DE, Orkin SH. Nat Genet. 2021 Apr;53(4):511-520. doi: 10.1038/s41588-021-00798-y. Epub 2021 Mar 1. PubMed
CRISPRi/a Screening with Human iPSCs. Nishiga M, Qi LS, Wu JC. Methods Mol Biol. 2021;2320:261-281. doi: 10.1007/978-1-0716-1484-6_23. PubMed
Locus-Specific DNA Methylation Editing in Melanoma Cell Lines Using a CRISPR-Based System. Smith J, Banerjee R, Waly R, Urbano A, Gimenez G, Day R, Eccles MR, Weeks RJ, Chatterjee A. Cancers (Basel). 2021 Oct 29;13(21). pii: cancers13215433. doi: 10.3390/cancers13215433. PubMed
Active demethylation upregulates CD147 expression promoting non-small cell lung cancer invasion and metastasis. Liao CG, Liang XH, Ke Y, Yao L, Liu M, Liu ZK, He L, Guo YX, Bian H, Chen ZN, Kong LM. Oncogene. 2022 Mar;41(12):1780-1794. doi: 10.1038/s41388-022-02213-0. Epub 2022 Feb 7. PubMed
Secretory defects in pediatric osteosarcoma result from downregulation of selective COPII coatomer proteins. Wood RK, Flory AR, Mann MJ, Talbot LJ, Hendershot LM. iScience. 2022 Mar 17;25(4):104100. doi: 10.1016/j.isci.2022.104100. eCollection 2022 Apr 15. PubMed
Inducible CRISPR activation screen for interferon-stimulated genes identifies OAS1 as a SARS-CoV-2 restriction factor. Danziger O, Patel RS, DeGrace EJ, Rosen MR, Rosenberg BR. PLoS Pathog. 2022 Apr 14;18(4):e1010464. doi: 10.1371/journal.ppat.1010464. eCollection 2022 Apr. PubMed

Associated Plasmids
Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer-promoter interactions. Wang K, Escobar M, Li J, Mahata B, Goell J, Shah S, Cluck M, Hilton IB. Nucleic Acids Res. 2022 Aug 12;50(14):7842-7855. doi: 10.1093/nar/gkac582. PubMed
Activation of stably silenced genes by recruitment of a synthetic de-methylating module. Chan WF, Coughlan HD, Chen Y, Keenan CR, Smyth GK, Perkins AC, Johanson TM, Allan RS. Nat Commun. 2022 Sep 23;13(1):5582. doi: 10.1038/s41467-022-33181-4. PubMed

Associated Plasmids
G-quadruplex-guided RNA engineering to modulate CRISPR-based genomic regulation. Liu X, Cui S, Qi Q, Lei H, Zhang Y, Shen W, Fu F, Tian T, Zhou X. Nucleic Acids Res. 2022 Oct 28;50(19):11387-11400. doi: 10.1093/nar/gkac870. PubMed
Extensive androgen receptor enhancer heterogeneity in primary prostate cancers underlies transcriptional diversity and metastatic potential. Kneppers J, Severson TM, Siefert JC, Schol P, Joosten SEP, Yu IPL, Huang CF, Morova T, Altintas UB, Giambartolomei C, Seo JH, Baca SC, Carneiro I, Emberly E, Pasaniuc B, Jeronimo C, Henrique R, Freedman ML, Wessels LFA, Lack NA, Bergman AM, Zwart W. Nat Commun. 2022 Nov 30;13(1):7367. doi: 10.1038/s41467-022-35135-2. PubMed
IMPDH inhibition activates TLR-VCAM1 pathway and suppresses the development of MLL-fusion leukemia. Liu X, Sato N, Yabushita T, Li J, Jia Y, Tamura M, Asada S, Fujino T, Fukushima T, Yonezawa T, Tanaka Y, Fukuyama T, Tsuchiya A, Shikata S, Iwamura H, Kinouchi C, Komatsu K, Yamasaki S, Shibata T, Sasaki AT, Schibler J, Wunderlich M, O'Brien E, Mizukawa B, Mulloy JC, Sugiura Y, Takizawa H, Shibata T, Miyake K, Kitamura T, Goyama S. EMBO Mol Med. 2023 Jan 11;15(1):e15631. doi: 10.15252/emmm.202115631. Epub 2022 Dec 1. PubMed
Mitotic clustering of pulverized chromosomes from micronuclei. Lin YF, Hu Q, Mazzagatti A, Valle-Inclan JE, Maurais EG, Dahiya R, Guyer A, Sanders JT, Engel JL, Nguyen G, Bronder D, Bakhoum SF, Cortes-Ciriano I, Ly P. Nature. 2023 Jun;618(7967):1041-1048. doi: 10.1038/s41586-023-05974-0. Epub 2023 May 10. PubMed
Multiplex single-cell chemical genomics reveals the kinase dependence of the response to targeted therapy. McFaline-Figueroa JL, Srivatsan S, Hill AJ, Gasperini M, Jackson DL, Saunders L, Domcke S, Regalado SG, Lazarchuck P, Alvarez S, Monnat RJ, Shendure J, Trapnell C. bioRxiv. 2023 Jun 14:2023.03.10.531983. doi: 10.1101/2023.03.10.531983. Preprint. PubMed
ASCT2 is the primary serine transporter in cancer cells. Conger KO, Chidley C, Ozgurses ME, Zhao H, Kim Y, Semina SE, Burns P, Rawat V, Sheldon R, Ben-Sahra I, Frasor J, Sorger PK, DeNicola GM, Coloff JL. bioRxiv [Preprint]. 2023 Oct 11:2023.10.09.561530. doi: 10.1101/2023.10.09.561530. PubMed
Enhancing prime editor flexibility with coiled-coil heterodimers. Mu S, Chen H, Li Q, Gou S, Liu X, Wang J, Zheng W, Chen M, Jin Q, Lai L, Wang K, Shi H. Genome Biol. 2024 Apr 26;25(1):108. doi: 10.1186/s13059-024-03257-z. PubMed

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