Cerulean(159-238) in pcDNAI/Amp
(Plasmid #60898)

• Purpose: This is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.
• Depositing Lab: Catherine Berlot
• Publication: Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27.

Backbone

• Vector backbone: pcDNA1/amp
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4800
• Total vector size (bp): 5020
• Modifications to backbone: BglII site introduced into the polylinker 3' to the BamHI site
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cerulean(159-238)
• Alt name: Fragment of Cerulean, a modified form of Cyan Fluorescent Protein
• Species: Aequorea victoria
• Insert Size (bp): 220
• Mutation: Contains N164H substitution
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cerulean(159-238) insert was amplified by PCR from monomeric Cerulean (David Piston, Vanderbilt University, Nashville, TN).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

N164H substitution added during PCR of Cerulean(159-238).

Reference for mCerulean:
Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol. 2004;22(4):445-9. PubMed PMID: 14990965.