Skip to main content
Addgene

CFP(159-238) in pcDNAI/Amp
(Plasmid #60896)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60896 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA1/Amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 5020
  • Modifications to backbone
    BglII site introduced into the polylinker 3' to the BamHI site
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CFP(159-238)
  • Species
    Aequorea victoria
  • Insert Size (bp)
    220
  • Mutation
    Asn-164 to His
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CFP(159-238) was amplified by PCR from ECFP (Clontech).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CFP(159-238) in pcDNAI/Amp was a gift from Catherine Berlot (Addgene plasmid # 60896 ; http://n2t.net/addgene:60896 ; RRID:Addgene_60896)

  • For your References section:

    Analysis of G protein betagamma dimer formation in live cells using multicolor bimolecular fluorescence complementation demonstrates preferences of beta1 for particular gamma subunits. Mervine SM, Yost EA, Sabo JL, Hynes TR, Berlot CH. Mol Pharmacol. 2006 Jul;70(1):194-205. Epub 2006 Apr 26. 10.1124/mol.106.022616 PubMed 16641313
Related items: