YFP(159-238) in pcDNAI/Amp
(Plasmid #60889)

• Purpose: This vector attaches YFP(159-238) to the N-terminus of a protein.
• Depositing Lab: Catherine Berlot
• Publication: Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10.

Backbone

• Vector backbone: pcDNA1/amp
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4800
• Total vector size (bp): 5020
• Modifications to backbone: BglII site introduced into the polylinker 3' to the BamHI site
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YFP(159-238)
• Species: Aequorea victoria
• Insert Size (bp): 220
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: YFP(159-238) was amplified by PCR from enhanced YFP (Clontech)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry