single polypeptide FPX biosensor for calcium ion
(Plasmid #60887)

• Purpose: FPX biosensor
• Depositing Lab: Robert Campbell
• Publication: Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.

Backbone

• Vector backbone: pcDNA3 (+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 8278
• Modifications to backbone: multi-cloning sites have been modified to put 5' XhoI and 3' HindIII sites in the backbone
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: single polypeptide FPX biosensor for calcium ion
• Alt name: ddRFP A-Calmodulin-ddFP B-M13-ddGFP A
• Alt name: dimerization dependent red or green fluorescent protein (ddRFP or ddGFP) copy A and copy B
• Species: Synthetic; synthetic construct
• Insert Size (bp): 2778
• GenBank ID: KM891585
• Promoter: CMV
• Tag / Fusion Protein:
  • HindIII site (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry