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Addgene

single polypeptide FPX biosensor for both caspase-3 and -8
(Plasmid #60886)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 60886 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3 (+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7720
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    single polypeptide FPX biosensor for caspase-3 and 8
  • Alt name
    ddRFP A-IETD-ddFP B-DEVD-ddGFP A
  • Alt name
    dimerization dependent red or green fluorescent protein (ddRFP or ddGFP) copy A and copy B
  • Species
    Synthetic; synthetic construct
  • Insert Size (bp)
    2220
  • GenBank ID
    KM891584
  • Promoter CMV
  • Tags / Fusion Proteins
    • NES sequence LALKLAGLDIGS (C terminal on insert)
    • HindIII site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 Promoter forward primer
  • 3′ sequencing primer BGH Reverse primers
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    single polypeptide FPX biosensor for both caspase-3 and -8 was a gift from Robert Campbell (Addgene plasmid # 60886 ; http://n2t.net/addgene:60886 ; RRID:Addgene_60886)

  • For your References section:

    Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange. Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. 10.1038/nmeth.3261 PubMed 25622108
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